Anti-Microbial Potential of Panacea (Nigella Sativa)

  • Atika Liaqat Lecturer of Pharmacy The University of Faisalabad, Faisalabad
  • Liaqat Ali Professor of Anatomy University Medical & Dental College, Faisalabad
  • Muhammad Awais Khalid Agriculture Officer, Pesticide Quality Control Laboratory, Punjab Agriculture Department, Kalashahkako
  • Faiqa Liaqat Demonstrator of Anatomy Aziz Fatima Medical & Dental College, Faisalabad
  • Faiza Liaqat Demonstrator of Pharmacology Independent Medical College, Faisalabad
Keywords: Antimicrobial potential, antibiotic discs, Ciprofloxacin, Multi drug resistant (MDR) pathogens

Abstract

Background: In the existing scenario, increasing drug cost, toxic effects and drug resistance or the main motivating factors for researchers to exploit the natural bioactive sources for safe and natural therapeutic as an alternative to antibiotics. Objectives: The main objective of the study is to find out the antimicrobial potential, its spectrum and magnitude in N.sativa. Study Design: Experimental/in vitro study. Duration: September, 2016 – February, 2017. Settings: Different departments (of School of Pharmacy, Pathology and Bio-chemistry of UM & DC), The University of Faisalabad. Methodology: Antibiotics assay is performed by using the disc diffusion methods. N.sativa extracts are prepared and from these sensitivity discs are prepared. Standard size inoculum is also prepared. Then by using standard disc diffusion method the zones of inhibitions produced by N.sativa extracts are measured and recorded in mm and is compared with positive and negative controls. Results: Spice extract tested against test organisms revealed antimicrobial potency with diameter of zone of inhibition (27.17±0.98mm - 31.33±0.21mm) while positive control manifested zone of inhibition (19.33±0.21 - 29.17±0.17mm). Ethanol extracts prepared by Soxhlet apparatus showed better results compared with extracts prepared by simple maceration process. Furthermore, overall ethanol extracts showed better results compared with the aqueous extracts. Conclusion: This study revealed the presence of natural bioactive compound(s) in N.sativa with highly significant broad spectrum antibacterial potential, even against multi resistant pathogens.

Published
2018-07-26
How to Cite
Liaqat, A., Ali, L., Khalid, M., Liaqat, F., & Liaqat, F. (2018). Anti-Microbial Potential of Panacea (Nigella Sativa). Annals of Punjab Medical College, 12(3), 202-206. https://doi.org/10.29054/apmc/2018.107